rabbit polyclonal anti apoa1  (Biosynth Carbosynth)

Journal: Journal of Proteome Research

Article Title: Cerebrospinal Fluid Proteomic Profiles in Patients with Postherpetic Neuralgia

doi: 10.1021/acs.jproteome.3c00547

Figure Lengend Snippet: Summary of involved processes among PLG, APOA1, and APOE in the process of PHN.

Article Snippet: The membrane then was blocked in TBST containing 10% nonfat milk powder for 1 h followed by the incubation at 4 °C overnight with the following primary antibodies: rabbit anti-PLG (1:500, BS9229, Bioworld Technology), rabbit anti-APOA1 (1:500, bs-0849R, Bioss), and rabbit anti-APOE (1:2000, ab183597, Abcam) were used.

Techniques:

Journal: Acta Pharmaceutica Sinica. B

Article Title: PPAR α affects hepatic lipid homeostasis by perturbing necroptosis signals in the intestinal epithelium

doi: 10.1016/j.apsb.2024.08.021

Figure Lengend Snippet: Pparα deficiency in intestinal epithelium increases the translocation of gut-derived antigens into the liver. (A) Intestinal permeability assessment (FITC-dextran, 4 kD) in 8-week-old mice ( n = 10). (B) Relative mRNA levels of Zo-1 and Cldn8 in the ileum from 8-week-old mice ( n = 5). (C) The relative proportion of bacterial species in the cecum content by 16S rRNA gene sequencing ( n = 6). (D) Bugbase phenotypic prediction of gut microbiota in 8-week-old mice ( n = 6). (E) The mRNA and protein levels of PV1 in the ileum of 8-week-old mice ( n = 5). (F) Transmission electron microscopy images of the diaphragm (red star) in the capillaries from ileum sections in 24-week-old mice ( n = 3). (G) Representative images of fluorescence microscopy and transmission electron in 8-week-old mice treated with FITC-LPS (green) or Au-LPS ( n = 3–5). (H) Portal HDL-C levels in 8-week-old mice ( n = 10). (I) The protein levels of APOA1 and ABCA1 in the ileum of 8-week-old mice ( n = 5). (J) Relative mRNA levels of Apoa1 , Pon1 , and Pon3 in the ileum of 8-week-old mice ( n = 5). (K) Serum APOA1 levels in 8-, 16- and 32-week-old mice ( n = 8–10). (L) Serum APOA1 levels in 8-week-old mice exposed to HFCS for 14 days ( n = 8). (M) Serum APOA1 levels in 16-week-old Pparα Δhep mice ( n = 10). (N) Schematic representation of a cocktail of broad-spectrum antibiotics (Abx) experimental design. (O) Representative images stained with H&E and Oil Red O, and immunofluorescent staining for F4/80 (red) in the liver from 8-week-old mice treated with Abx ( n = 5). (P) Triglyceride in serum and liver treated with Abx ( n = 10). (Q) Relative mRNA levels of genes related to triglyceride accumulation in the liver from 8-week-old mice treated with Abx ( n = 5). (R) Hepatic levels of cytokines from 8-week-old mice treated with Abx ( n = 5). (S) Protein levels of F4/80 in the liver of 8-week-old mice treated with Abx ( n = 3). (T) Relative mRNA levels of F4/80, Clec4f , and Cd14 in the liver of 8-week-old mice treated with Abx ( n = 5). LD: lipid droplet; M: mitochondria; FITC-LPS: fluorescein isothiocyanate (FITC)-LPS; Au-LPS: LPS-gold-complexes. Data are shown as the mean ± SD. An unpaired two-tailed Student's t -test; ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: These samples were incubated overnight with a polyclonal rabbit anti-mouse F4/80 (1:1000, #A1256, ABclonal), polyclonal rabbit anti-mouse APOA1 (1:2000, #A14211, ABclonal), polyclonal rabbit anti-mouse ABCA1 (1:1000, #NB400-105, Novus Biological, Centennial), monoclonal rabbit anti-mouse CD14 (1:1000, #A19011, ABclonal), or polyclonal rabbit anti-mouse PV1 (1:2000, #A15906, ABclonal).

Techniques: Translocation Assay, Derivative Assay, Permeability, Sequencing, Transmission Assay, Electron Microscopy, Fluorescence, Microscopy, Staining, Two Tailed Test

Journal: Journal of Traditional and Complementary Medicine

Article Title: Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression

doi: 10.1016/j.jtcme.2023.02.010

Figure Lengend Snippet: Primer sequences for qRT-PCR.

Article Snippet: Samples were incubated with polyclonal rabbit anti-NR1H4 primary antibody (1:200, bs-12867, Bioss, Beijing) or polyclonal rabbit anti-APOA1 primary antibody (1:200, bs-0849R, Bioss, Beijing) at 4 °C overnight.

Techniques:

Journal: Journal of Traditional and Complementary Medicine

Article Title: Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression

doi: 10.1016/j.jtcme.2023.02.010

Figure Lengend Snippet: YCHT increased the NR1H4 and APOA1 gene expression. (A) Relative mRNA expression of ADIPOQ. (B) Relative mRNA expression of PPARG. (C) Relative mRNA expression of IGF1. D, Relative mRNA expression of PPARA. (E) Relative mRNA expression of MTHFR. (F) Relative mRNA expression of SERPINE1. (G) Relative mRNA expression of NR1H4. (H) Relative mRNA expression of APOA1. (I) Relative mRNA expression of APOA2. ∗p < 0.05, ∗∗p < 0.01; n = 3–4.

Article Snippet: Samples were incubated with polyclonal rabbit anti-NR1H4 primary antibody (1:200, bs-12867, Bioss, Beijing) or polyclonal rabbit anti-APOA1 primary antibody (1:200, bs-0849R, Bioss, Beijing) at 4 °C overnight.

Techniques: Expressing

Journal: Journal of Traditional and Complementary Medicine

Article Title: Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression

doi: 10.1016/j.jtcme.2023.02.010

Figure Lengend Snippet: YCHT restored NR1H4 and APOA1 expression of NAFLD. (A) NR1H4 protein expression pattern. (B) APOA1 protein expression pattern. ∗p < 0.05; n = 3–4.

Article Snippet: Samples were incubated with polyclonal rabbit anti-NR1H4 primary antibody (1:200, bs-12867, Bioss, Beijing) or polyclonal rabbit anti-APOA1 primary antibody (1:200, bs-0849R, Bioss, Beijing) at 4 °C overnight.

Techniques: Expressing

Journal: Journal of Traditional and Complementary Medicine

Article Title: Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression

doi: 10.1016/j.jtcme.2023.02.010

Figure Lengend Snippet: NR1H4 and APOA1 localization pattern in the liver. (A) NR1H4 expression pattern in the liver. (B) APOA1 expression pattern in the liver. Scale bar: 50 μm; image at 400 × .

Article Snippet: Samples were incubated with polyclonal rabbit anti-NR1H4 primary antibody (1:200, bs-12867, Bioss, Beijing) or polyclonal rabbit anti-APOA1 primary antibody (1:200, bs-0849R, Bioss, Beijing) at 4 °C overnight.

Techniques: Expressing